Aim: To compare the antimicrobial activity of different concentrations of Chamomile Extract and Chlorhexidine gel against Candida albicans and Enterococcus faecalis.

Methods: We evaluated the antimicrobial activity of 15%, 25% Matricaria chamomilla in aq. base and 2% chlorhexidine gel against C. albicans and E. faecalis strains by agar diffusion test. Vancomycin was used as the positive control for E. faecalis and fluconazole for C. albicans. The agar plates were incubated at 37 °C for 48 h after which the zone of inhibition were measured separately for each material.

Results: The mean values of microbial growth inhibition against C. albicans produced by 25% M. chamomilla was 25.25±0.478, by 2% Chlorhexidine was 34.82±0.825. The mean values of microbial growth inhibition against E. faecalis produced by 25% M. chamomilla was 21.27±0.652, by 2% Chlorhexidine was 25.84±0.494. 2% Chlorhexidine gel showed the strongest antimicrobial action, producing the largest zones of inhibition, followed by 25% Matricaria, whereas 15% Matricaria did not show any antimicrobial activity.

Conclusion: It is clear that Matricaria chamomilla in concentrations more than 15% aq. base is effective and provides a good alternative as an antimicrobial agent in dental practice.