Introduction: Chronic renal failure is defined as the progressive and irreversible decline of the glomerular filtration rate (GFR), and gradual loss of kidney function over time, causing to an increase of serum creatinine and blood urea nitrogen levels leading to end-stage renal disease.

Aims and Objectives: The aim of the study was to estimate and compare the salivary and serum urea, creatinine, and PH in pre-dialysis and post-dialysis state in end-stage renal disease patients.

Materials and Methods: The sample comprised of patients with renal failure, who were undergoing hemodialysis at Government hospital in Nizamabad. The venous blood and saliva samples were collected from the patients during pre and post dialysis stages. Collected samples were immediately sent to the laboratory for biochemical examination of creatinine, urea, and Ph.

Results: Statistical analysis was done for the comparison of serum and salivary samples of all the three parameters in pre and post dialysis states using paired t-test in which gave a significant p-value of 0.0001.

Conclusion: Patients who are under hemodialysis show apparent oral and salivary changes. In our study, a significant correlation was obtained between salivary creatinine levels when compared to urea and PH thus salivary creatinine levels will help in diagnosing of patients with ESRD.