AUTHOR(S): Ruqshan Anjum MG, Sujatha I, Sharath Chandra SM

ABSTRACT:
This study was done to test the antimicrobial efficiency of four irrigating solutions such as chlorhexidine (CHX), Oxum, Ozonated water and normal saline, against the E. Faecalis microorganisms in root canals. Forty freshly extracted single-rooted human teeth were selected. 20 μL of the suspension was inoculated into each of 40 root canals. The specimens were incubated at 37 °C for 7 days. After 48 h of incubation at 37 °C, microbial growth was verified, confirming the contamination of the root canals. They were enlarged to a size 80 K-file (Maillefer). Instrumentation was followed by irrigation with 3 mL of the above irrigating solutions for each file used. After the instrumentation, the first sampling was done immediately followed by second sampling on 3 ^rd and third sampling on 7 ^th day from the root canals. Within the limitations of this study it was concluded that chlorhexidine significantly reduced the number of E. faecalis followed by oxum and ozonated water was observed.

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