AUTHOR(S): Dennis, Cut Nurliza and Wulandari Savitri

ABSTRACT:Porphyromonas gingivalis is one of the bacterial that often found in primary endodontic infection and able to form biofilm. Root canal irrigation is very important step to eliminate bacterial and biofilm, but currently there is no ideal irrigant solution. Avocado seed (Persea americana Mill.) is one of the herbal product which has antibacterial effect and able to developed as an alternative irrigants. The purpose of this study is to know the antibacterial effect of ethanol extract of the avocado seed (Persea americana Mill.) by finding the minimum inhibitory concentration (MIC) and minimum bactericidal concentration. This study design is posstest only control group and that collected by doing the experiments. 300 gram of avocado seed (Persea americana Mill.) simplicia is being macerated with 70% alcohol in 15 minutes, then percolated until percolator fluid become clear, after that evaporated with rotary evaporator till get 60 gram of viscous extract. MIC value determination is done using dilution method by dilute the extract in Trypticase Soy Broth (TSB) with concentration 100%, 50%, 25%, 12,5%, 6,25%, and 3,125%. 4 ml of each concentrations is taken and added 100 µl of bacterial suspension, vortexed and incubated in temperature 37○C for 24 hours. The turbidity of each tube is observed visually and compared with Mc Farland control. The tube that start to look clear is MIC. Next, each concentration vortexed and took 100 100 µl, dropped in petri then poured it in Trypticase Soy Agar (TSA) media and replicated into 4 samples, incubated in temperature 37○C for 24 hours, then counting the number of bacterial colony with Pour Plate method to determine MBC value. In dillution test the tube start to look clear in concentration 50%, in bacterial colony counting using Pour Plate method showed the bacterial growth in concentration 50% with average 1,75.107 CFU/ml.Further study was done to determine MBC value and found concentration 60%. Kruskal-Wallis test showed that avocado seed (Persea americana Mill.) ethanol extract had antibacterial effect against Porphyromonas gingivalis (p=0,000) and Mann-Whitney test showed there are significant differentiation between concentration 50% with 100%, 80%, and 60%. The conclusion of this study is ethanol extract of the avocado seed (Persea americana Mill.) had antibacterial effect against Porphyromonas gingivalis with MIC value in concentration 50% and MBC value in concentration 60%.

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