Aims: The aim of the study was to evaluate the relationship between GCF volumes with different periodontal status at various tooth site locations.

Settings and Design: This was a randomized clinical trial. GCF was obtained from the gingival crevice in 32 tooth sites of 12 individual participants.

Methods and Material: Clinical parameters such as gingival index (GI) & probing depth (PD) were recorded. GCF was collected with absorbent paper strips from the gingival crevice of incisor, canine, premolar and molar. These strips were placed in periotron 8000 device (Oraflow Inc) to quantify GCF volume.

Statistical analysis used: One way ANOVA was carried out for comparison of mean GCF volume among subgroups of GI and PD at various tooth site location.

Results: GCF volumes collected at various anatomical sites were varying in amount. GCF volume increases with the increase in GI and PD.

Conclusions: This study concludes that there is variation in an increase in GCF volume collected from various tooth sites of different periodontal status.