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International Journal of Applied Dental Sciences
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ISSN Print: 2394-7489, ISSN Online: 2394-7497
ICV 2018: 74.52

International Journal of Applied Dental Sciences

2018, Vol. 4 Issue 2, Part D

Role of common fig and olive oil on the Nitropropane-induced submandibular gland changes: Role of IL1β, IL6 and TNFα

AUTHOR(S): Atef I Ahmed and Badawy M Abd-El-Hamied
ABSTRACT:Objectives: 2-Nitropropane (2-NP) is a rat liver carcinogen. Animals chronically exposed to 2-nitropropane by inhalation, liver and pulmonary effects have been observed. The aim of this study was to verify if common fig and olive oil have a synergistic effect on the nitropropane alterations on the IL-1β, TNFα and IL 6 in submandibular gland of Mice.
Methods and materials: The present investigation was carried out on forty adult BALB/c male mice. The mice were divided randomly into 5 equal groups. Group I, a control group (n=8), which were injected with vehicle alone (canola oil, 5 mL/kg). The other four groups (n = 32) by intraperitoneal (i.p.) injection of 2- Nitropropane (100 mg/kg body weight dissolved in canola oil) 2 time/week for 4 weeks. At the same time of 2- NP injection, mice of the latter three groups were orally drinking water supplemented with fig extract, olive oil and mixture of fig and olive oil 50 (Mg/Ml)/ 4 weeks respectively. The gene expression levels of tumor necrosis factor-alpha (TNF-a), interleukin 1-β (IL-1β), and interleukin 6 (IL-6) were tested in submandibular gland of mice by RT-qPCR.
Results: The gene expression analysis showed that the 2-nitropropane caused statistically significant upregulation of TNF-α, IL-1β, and IL-6 compared to the untreated control group. Elevated expression of IL-1β and IL-6 were significantly downregulated by olive oil and fig treatment. Co-administration of fig with olive mixture markedly down-regulate the gene expression analysis TNF-α, IL-1β, and IL-6 compared to the 2-NP treated group.
Conclusions: The findings conclude that olive oil and fig extract may attenuate the alterations of 2-NP by down-regulating proinflammatory cytokines IL-1β, IL6 and TNF α.
Pages: 200-205  |  564 Views  6 Downloads
How to cite this article:
Atef I Ahmed, Badawy M Abd-El-Hamied. Role of common fig and olive oil on the Nitropropane-induced submandibular gland changes: Role of IL1β, IL6 and TNFα. Int J Appl Dent Sci 2018;4(2):200-205.
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