Anti-infective periodontal treatment includes mechanical pocket debridement (scaling and root planing) to remove dental calculus and administration of antimicrobial agents. Antimicrobial agents may have side effect such as microbials resistances to antimicrobials. The use of herbal therapy has been considered as alternative of antimicrobial therapy. Citrus (Citrus aurantifolia (chrism.) Swingle) has been shown to have antimicrobial, anti-oxidants and anti-inflammatory effect.
Objective: The aim of this study was to know and to analyze the effectiveness of citrus peel extract to inhibiti the growth of some periodontal pathogens bacteria in vitro.
Methods: Citrus peel was extracted by percolation method using ethanol, n-hexane and ethyl acetate solvent to produce a viscous extract citrus peel. The extract was diluted to a concentration of 25%, 12%, 6.25% and 3.125%. Each concentration was tested for its antimicrobial avtivity effectiveness against A. actinomycetemcomintans, P.gingivalis, F.nucleatum. The effectiveness of the extract was shown as inhibition zone on BHI broth media.
Results: Citrus peel extract of ethanol at a concentration of 25% has the strongest antibacterial effectiveness against the three bacteria compared to solvents, with a diameter of inhibition zone against A. actinomycetemcomintans 16.05 mm, P.gingivalis and F.nucleatum 12.75 mm and 11.67 mm. There are statistically significant differences among extract concentration of 25%, 12%, 6,25% and 3,125% and citrus peel extract (p<0.05).
Conclusion: citrus peel extract has antibacterial effect in inhibiting bacterial growthA. actinomycetemcomintans, P.gingivalis, F.nucleatum.