International Journal of Applied Dental Sciences
2018, Vol. 4 Issue 3, Part F
The differential electrophoretic patterns of statherin and histatins in caries-active and caries-free children
AUTHOR(S): Meltem Bakkal and Betul Kargul
The present study aimed to reveal the possible different protein profiles of children who had caries-active and caries-free deciduous dentition. Salivary parameters including salivary pH, flow rate, the concentrations of calsium, phosphate and total protein, were also evaluated to see the possible changes in case of dental caries.
Materials and methods: Eight subjects were participated in ‘caries-active group’ and the other 10 subjects were in ‘caries-free group’. Salivary parameters including salivary pH, flow rate and the concentrations of calcium, phosphate and total protein were evaluated in both groups and the electrophoretic patterns for Statherin and Histatins in the groups were analysed by using Discontinuous Native Polyacrylamide Gel Electrophoresis and Basic Gel Electrophoresis; respectively.
Results: The slight differences were observed on the salivary parameters of the groups (p>0.05). Salivary proteins were identified according to their relative mobility in gel and stain patterns. A total of 7 Statherin (38.8%) and 3 Histatin1 (16.6%) bands were counted on the gels. All 3 Histatin1 bands were seen in only caries-free samples meanwhile 6 of 7 Statherin bands (33.2%) were in caries-free samples, as well.
Conclusion: These preliminary findings report that satatherin and histatin1 had different electrophoretic patterns in children with different caries status. Additional studies can provide further evidence concerning the role of each salivary proteins in modifying risk for dental caries.
Pages: 365-370 | 1069 Views 26 Downloads
How to cite this article:
Meltem Bakkal, Betul Kargul. The differential electrophoretic patterns of statherin and histatins in caries-active and caries-free children. Int J Appl Dent Sci 2018;4(3):365-370. DOI: https://doi.org/10.22271/oral.2018.v4.i3f.05