Methodology: 20 healthy patients satisfying the criteria were recruited for the study. Among these patients group I consisted of 10 patients being treated with Self-ligating Labial prescription and group II consisted of 10 patients being treated with Self-Ligating Lingual prescription. Salivary samples of all the 20 subjects were collected at various intervals. All the collected sample were cultured and the microbes were isolated and identified according to their cultural Characteristics and Biochemical reactions at T0 (starting of treatment), T1 (3 months after treatment) and T2 (6 months after initial treatment) intervals.

Conclusion: The count of staphylococcus aureus increased progressively during the treatment and was seen more in Self-Ligating Lingual orthodontic appliance than Self-ligating Labial orthodontic appliance.