To evaluate mineral content of the apical extruded material during irrigation with distile water, Sodium hypochlorite (NaOCl), Ethylenediaminetetraacetic acid (EDTA), QMix 2-in-1 solution, and preparation with Reciproc systems.
Methodology: Single rooted eighty eight teeth were randomly assigned to eight groups (n=11). Irrigation process was performed with distile water, NaOCl, EDTA, QMix 2-in-1 solutions during instrumentation with Reciproc and Reciproc blue systems. Apical extruded material was collected into the Eppendorf tubes, then maintained to Vortex for homojenization. The tubes were stored in an incubator for evaporation. The amount of apical extruded material was calculated by substracting pre-instrumentation and post-instrumentation weight of the tubes. An amount of homojenized debris was spreaded to the analysis platform and analysed for determining the relative amounts of elements. The Friedman test was used for the repeated measures of multiple groups (mean, standard deviation), Kruskal Wallis test for subgroup comparison
Results: In distile water group Reciproc Blue instrumentation associated with significantly high extruded debris than Reciproc instrumentation (p = 0.001), where as no significant difference was found between the Reciproc and Reciproc Blue instrumentation in the NaOCl, EDTA, Qmix groups. In the Reciproc and Reciproc Blue subgroup, the percentage weight of Ca and P, in the Distilled water group was statistically significantly higher than those of the NaOCl, EDTA and QMIX groups (p = 0.0001)
Conclusions: Reciproc systems and the irrigating solutions caused extrusion of material. EDTA irrigation produced higher extruded material than the other irrigants however it extruded lower Ca and P levels.