Aims: To determine the microleakage of the 6th, 7th, and 8th generation dentin bonding agents.

Materials and Methods: Standardized Class II cavities were prepared on the mesial and distal aspects of Forty-five extracted human molars. According to the generation of bonding agent used, the samples were divided into three groups. 6th generation bonding agent (Parabond) is bonded in group I, 7th generation (One coat 7) in group II and 8th generation (G premio bond) in group III respectively. Nano-ceramic composite (Ceram X) is restored in all the prepared cavities. The samples were then thermocycled between 5 and 55 ± 2°C for 100 cycles and immersed in 2% methylene blue for 48 h, Sectioning is done for evaluation of microleakage under a stereomicroscope in 20X magnification.)

Statistical Analysis Used: The data was statistically analyzed using analysis of variants (ANOVA), and post hoc analysis based on Bonferroni test was applied to compare the various groups.

Results: The highest value of microleakage was in Group II (7th generation bonding agent) followed by Group I (6th generation bonding agent) and least in Group III (8th generation bonding agent).

Conclusions: Statistically significant results was seen in the 8th generation dentin bonding agents as least microleakage when compared to the 6th and 7th generation dentin bonding agents.