Methods: In the clinical setting, trial was conducted involving 18, 14-15 year old school children, randomly allocated into three interventional groups, Group A (5% Sodium fluoride varnish, Fluoritop-SR varnish), Group B (5% Sodium fluoride - Tricalcium phosphate varnish, Clinpro White varnish) and Group C (5% Sodium fluoride- amorphous calcium phosphate-casein phosphopeptide varnish, MI Varnish) to receive the varnish application. Saliva samples were collected at the end of one hour after varnish application for S Mutans count estimation. Student’s paired ‘t’ test and One way-ANOVA tests were used for statistical analyses.

Results: Significant reductions in salivary streptococcus mutans count at one hour was achieved with application of B & C varnishes compared to varnish A.

Conclusion: amorphous calcium phosphate-casein phosphopeptide and tricalcium phosphate varnish groups exhibited better antimicrobial property against streptococcus mutans compared to Sodium fluoride varnish at one-hour post intervention.