Aim: of this study is evaluation of the anti-microbial properties of prepared herbal solution on dental impressions with irreversible hydrocolloid. The study is an ex-vivo comparative study.

Material and methods: includes Herbal ingredients: Neem, Tulsi, Reetha, Aloe vera, Neem seed oil, Ginger oil, Peppermint oil, Gum acacia, and Rock salt were used to prepare herbal disinfectant solution. Preparation of this disinfectant solution includes extract preparation by reflux method, filtration, evaporation and homogenization. Minimum inhibitory concentration of this solution was determined and in-vitro antimicrobial properties were checked with disc diffusion method. To check whether the anti-microbial property of herbal solution was useful in dentistry, 10 sectional maxillary impressions of left side were made with irreversible hydrocolloid. In the molar area each impression was cut in two halves. One half was washed with water and other half was sprayed with herbal solution. Impressions were kept for 2 to 3 minutes and then placed in inoculated agar plates. Incubation of agar plates was done for 24 hours and checked for bacterial growth. Mann Whitney ‘U’ test and Chi Square test were used to evaluate the data.

Results: found that Agar plates having sectioned alginate impression sprayed with disinfectant herbal solution showed less bacterial growth compared with the agar plates having alginate washed with water. To conclude Herbal disinfectant solution is effective in controlling the bacterial growth on impressions with irreversible hydrocolloid