AUTHOR(S): Minha Majeed Kak, Arya Jyoti, Anurag Nath, Priyanka Rastogi and Sachin Kumar

ABSTRACT:Background: Dental students routinely use extracted human teeth for preclinical and clinical procedures thus require constant handling of the same. Extracted teeth can be potent sources of blood borne pathogens and other infections thus require proper disinfection of them. Centers for Disease Control and Prevention (CDC) has adopted guidelines for infection control of extracted teeth used for research and teaching, requiring that teeth be sterilized before use. Many methods of disinfection have been proposed in literature having their own advantages and disadvantages.
Aim: To asses vinegar as disinfectant of extracted human teeth and its effectiveness in sterilization.
Material and Methods: A total of 50 extracted non-carious human teeth were sterlised both by physical methods as by Autoclaving, boiling and microwaving and chemical methods by keeping in seven disinfectant media—10% formalin, 6.5% hydrogen peroxide, 5% sodium hypochlorite, 70% alcohol, vinegar, Betadine and normal saline. Later, teeth from each group were placed individually in separate test tubes containing 10 ml of Brain heart infusion at 37°C for 48 h to observe the evidence of growth of microorganisms.
Results: Results were analyzed using Kruskal-Wallis test. Vinegar, 10% Formalin, Betadine and 6.5% Hydrogen peroxide were effective. The results were statistically significant with Kruskal-Wallis test value 30.031 with p-value <0.001.
Conclusion: Vinegar can be used as an effective non-hazardous disinfectant for extracted human teeth.

DOI: https://doi.org/10.22271/oral.2021.v7.i4c.1362

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