Vol. 8, Issue 2, Part I (2022)
Correlation of the SNP’s of fgfr1 and fgf10 with non-syndromic cleft lip with or without cleft palate in Indian population by DNA sequencing method
Author(s):
Dr. Sapna Kumari, Dr. Prashanth CS, Dr. Amarnath BC, Dr. Dharma RM, Dr. Akshai Shetty and Dr. Manjunath Hegde
Abstract:
Nonsyndromic cleft lip & palate is a congenital deformity of multifactorial origin.
Various etiologic candidate genes have been reported with conflicting results, according to the race and population studied. Numerous efforts have been made to understand the etiology of cleft/palate so as to predict its occurrence and thus enable its prevention. The FGF regulation pathway also plays a major role in craniofacial development. FGFR1, FGFR2, FGFR3, FGF10 and FGF18 are expressed during the various stages of secondary palate development. Thus their inactivation leads to cleft palate.
This study was done to evaluate the association of FGFR1 and FGF10 gene variants with nonsyndromic cleft lip and palate. DNA samples of 25 subjects with nonsyndromic cleft lip and palate and 25 unrelated controls, collected from the department were used for the study.
The extracted DNA samples were subjected to Polymerase chain reaction later subjected to DNA sequencing. The results were documented in the form of electropherogram.
The results indicated that for FGFR1 gene (rs13317), genotype CC (P=0.02) was statistically significant and FGF 10 gene (rs1448037), genotype AA (P< 0.001) was highly statistically significant. Thus suggesting a strong association between FGFR1 and FGF10 with NSCLP.
Various etiologic candidate genes have been reported with conflicting results, according to the race and population studied. Numerous efforts have been made to understand the etiology of cleft/palate so as to predict its occurrence and thus enable its prevention. The FGF regulation pathway also plays a major role in craniofacial development. FGFR1, FGFR2, FGFR3, FGF10 and FGF18 are expressed during the various stages of secondary palate development. Thus their inactivation leads to cleft palate.
This study was done to evaluate the association of FGFR1 and FGF10 gene variants with nonsyndromic cleft lip and palate. DNA samples of 25 subjects with nonsyndromic cleft lip and palate and 25 unrelated controls, collected from the department were used for the study.
The extracted DNA samples were subjected to Polymerase chain reaction later subjected to DNA sequencing. The results were documented in the form of electropherogram.
The results indicated that for FGFR1 gene (rs13317), genotype CC (P=0.02) was statistically significant and FGF 10 gene (rs1448037), genotype AA (P< 0.001) was highly statistically significant. Thus suggesting a strong association between FGFR1 and FGF10 with NSCLP.
Pages: 613-620 | 1096 Views 307 Downloads
How to cite this article:
Dr. Sapna Kumari, Dr. Prashanth CS, Dr. Amarnath BC, Dr. Dharma RM, Dr. Akshai Shetty and Dr. Manjunath Hegde. Correlation of the SNP’s of fgfr1 and fgf10 with non-syndromic cleft lip with or without cleft palate in Indian population by DNA sequencing method. Int. J. Appl. Dent. Sci. 2022;8(2):613-620. DOI: 10.22271/oral.2022.v8.i2i.1565
