Vol. 9, Issue 2, Part B (2023)
Effect of glycolic acid as single irrigant versus sodium hypochlorite and EDTA on enterococcus faecalis count and smear layer removal in single-rooted teeth (an in vitro study)
Author(s):
Hager Salah El-desoki, Hany Samy Sadek and Dina Ahmed Morsy
Abstract:
Aim: The study aimed to compare 10% Glycolic acid as a single irrigant versus 2.5% sodium hypochlorite followed by 17% Ethylenediaminetetraacetic acid (EDTA) in terms of E. faecalis count reduction and smear layer removal in single-rooted teeth.
Methodology: Thirty human extracted mandibular premolars were prepared and inoculated with E. faecalis for one week to evaluate E. faecalis count reduction. Teeth were randomly assigned to two groups (n = 15) according to the irrigation protocol used. Group I: intervention (10% Glycolic acid) and Group II: Control group (Irrigation using 2.5% NaOCl followed by 17% EDTA). After 24 hours of incubation, bacterial count reduction using colony forming units CFUs/ml was determined. For evaluating the ability of irrigants to remove the smear layer, another twenty-two mandibular premolars were chosen and randomized into two groups (n = 11). During “chemo-mechanical†preparations, the root canals were irrigated with different irrigation protocols according to each group. Then, the roots were split longitudinally in the bucco-lingual direction into two halves. A smear layer assessment was conducted on the “coronal, middle, and apical thirds†of the root canal using an Environmental Scanning-Electron Microscope (ESEM) at 1000x magnification.
Results: Regarding E. faecalis count reduction, there was “no significant†difference between the two groups (p = 0.14). Regarding the ability to remove the “smear layerâ€, there was no significant difference between the two groups at coronal, middle and apical. While comparing smear layer scores within the same group, the “apical third†showed a significantly higher smear layer score in the two groups.
Conclusion: Glycolic acid can be used as a single irrigant owing to its antibacterial efficacy and the ability to remove the smear layer.
Pages: 109-118 | 869 Views 344 Downloads
How to cite this article:
Hager Salah El-desoki, Hany Samy Sadek and Dina Ahmed Morsy. Effect of glycolic acid as single irrigant versus sodium hypochlorite and EDTA on enterococcus faecalis count and smear layer removal in single-rooted teeth (an in vitro study). Int. J. Appl. Dent. Sci. 2023;9(2):109-118. DOI: 10.22271/oral.2023.v9.i2b.1720