Materials and Method: This study was carried out on 30 permanent molar teeth of patients aged between 6-9 years. The clinical examination of all patients included visual inspection with the aid of a dental mirror, probe, explorer, cotton rolls, and radiographic imaging. Sufficient lighting was also utilized during the examination. The study was divided into three groups. The procedure for the application of pit and fissure remains the same. However, group A was performed under naked eyes, group B under magnified loupes, and group C under a microscope. After the procedure teeth were analyzed for retention.

Statistical Analysis Uses: Statistical analysis used was Rydges Criteria with a comparison of results from the same.

Results: Observations vary greatly when the sealant is applied clinically vs. with loupes and microscopes. Furthermore, the results demonstrate that bubble formation was among the highly significant criteria on application with naked eyes compared to the microscope and magnifying loupes, which didn’t illustrate the same.

Conclusion: The result of this research illustrates that bubble formation on applying pit and fissure sealant suggests that microorganisms could still penetrate the area of the pit and fissure, which might cause dental decay later in life. Teeth with partially retained sealants are more susceptible to developing caries lesions than those with fully covered pits and fissures because particles and food can accumulate in the uncovered areas.