In vitro analysis of antimicrobial activity in exposed collagen membranes applying chitosan-metronidazole in topical gel

Dora María López Trujillo; Dr. María Fernanda Mendoza Durán; Juan Israel Alvarado Pérez; María de los Ángeles Pietschmann Santamaría; Lizeth Alejandra Reyes Alvarado; Gilberto Zataraín Hernández; Martha Margarita Aguado Arzola

doi:10.22271/oral.2023.v9.i2a.1713

In vitro analysis of antimicrobial activity in exposed collagen membranes applying chitosan-metronidazole in topical gel

AUTHOR(S): Dora María López Trujillo, Dr. María Fernanda Mendoza Durán, Juan Israel Alvarado Pérez, María de los Ángeles Pietschmann Santamaría, Lizeth Alejandra Reyes Alvarado, Gilberto Zataraín Hernández and Martha Margarita Aguado Arzola

ABSTRACT:

Guided Bone Regeneration through the use of a membrane has been a Gold standard to obtain the best results, in turn, being a sensitive technique whose main complication is its exposure and infection by pathogenic periodontium bacteria.

Objective: To observe the antimicrobial effect of topical chitosan-metronidazole on Porphyromona gingivalis.

Materials and Methods: Topical Chitosan-Metronidazole was prepared with which it is antimicrobial sensitivity was observed in the crop medium of MÃ¼ller-Hinton agar with the well diffusion method after having applied 50 microliters of P. gingivalis, 8mm was extracted with a punch and filled with chitosan-metronidazole, the inhibitory halo was analyzed to obtain results.

Results: The inhibitory halo was observed and measured in the two samples which is 20 mm for both, obtaining a sensitive result.

Conclusion: Topical chitosan-metronidazole was shown to have a good effect on P. gingivalis.

DOI: https://doi.org/10.22271/oral.2023.v9.i2a.1713

Pages: 54-59 | 348 Views 105 Downloads

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In vitro analysis of antimicrobial activity in exposed collagen membranes applying chitosan-metronidazole in topical gel

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