Aim: The objective of this study was to determine and compare the Push-out bond strength of AH Plus sealer of root canal following prior application of calcium hydroxide (Ca[OH]₂) paste and Nano-silver gel as intracanal medication in single-rooted human extracted maxillary incisors.

Methodology: Using of ProTaper System up till master apical file # F5 have been carried out to prepare root canals of 30 human extracted maxillary incisors. According to the intracanal medication applied, division of teeth were carried out in random manner into three groups. Group I: Control group (No intracanal medication), Group II: Nano-silver gel and Group III: Calcium hydroxide Ca(OH)₂. Three weeks later, the elimination process of the intracanal medications were achieved by using passive ultrasonic irrigation of 5 mL 2.5% NaClO, in addition to that, a 17% (Ca[OH EDTA was used in the step of final flush for duration of 60 seconds succeeded by 5 mL of distilled water. Using the master cone #F5 and AH plus sealer, the single cone approach was used to fill the canals. After 1 week, the universal testing machine was implemented to test the push out-bond strength of the samples, which was performed for a 2-mm-thick of coronal, middle and apical thirds of each root.

Results: A remarkable difference was evident between the control group and the comparison groups (p < 0.001). Within the control group, the greatest value was evident (4.85±1.65), followed by calcium hydroxide (2.11±1.17), meanwhile the lowest value was discovered in nano-silver (1.41±1.08). Post hoc pairwise comparisons demonstrated a remarkably higher value in the control group than the rest of groups (p < 0.001) with no statistically remarkable difference between calcium hydroxide and Nano-silver group.

Conclusion: Prior calcium hydroxide and nano-silver placement seemed to decrease the push-out bond strength of epoxy resin-based Ah plus sealer.