A glide path file may contribute to extruding contaminated debris during the first treatment phase. Using rotary techniques rather than manually operated instruments is thought to decrease the amount of extruded debris and hence post-operative pain levels.

Aim: To compare the effect of using Neoniti GPS rotating glide path file in comparison to manual stainless-steel K-files on post-operative pain, in asymptomatic nonvital mandibular molars.

Methods: Thirty nonvital asymptomatic mandibular permanent molars were randomly allocated into two equal groups (n=15). In the control group, glide path preparation was achieved using manual stainless-steel K-files (#10, 15). In the experimental group, a glide path was created using Neoniti GPS path file. Standardized cleaning and shaping procedures were performed in both groups.

Results: Neoniti GPS for glide path preparation resulted in significantly less post-operative pain incidence at 6 h (p= 0.039) and was associated with significantly lower post-operative pain levels at 12 h (p=0.047). No other significant difference was found between the two groups at 24, 48 and 72 h.

Conclusions: Glide path preparation using Neoniti GPS rotary file decreased post-operative pain incidence after 6 h and reduced pain intensity after 12 h compared to manual glide path preparation.