Aim: This study aims to quantify and evaluate Salivary IgA levels in Carious Individuals pre and post-application of Fluoridated, Nano-hydroxyapatite and CPP-ACP toothpaste.

Materials and Methodology: In aggregate, 90 patients were selected randomly from the outpatient department. DMFT and DMFS indices were performed prior to patient selection. After completion of the analysis, 30 patients having a moderate score were selected. A saliva sample was collected before the commencement of the study and was stored. The individuals were then instructed to use different kinds of toothpaste for the next 15 days based on the groups.

They were randomly divided into the following groups based on toothpaste application (n=10):

Group 1: Fluoridated Toothpaste

Group 2: CPP-ACP Toothpaste

Group 3: Nano-Hydroxyapatite Toothpaste

The patients were recalled after 30 days of toothpaste application. The post-application salivary samples were analysed for IgA levels and quantified using ELISA Test.

The Intra-group and Inter-Group mean distribution of salivary IgA levels resulting from the ELISA Test were examined using One-way analysis of variance (ANOVA) in conjunction with Tukey’s Post-Hoc test. This analysis was employed for comparing multiple groups, and the SPSS software (Sigma Stats, Version 2.03, SPSS, Chicago) was utilized for this purpose.

Results: It was observed that among the different types of toothpaste, those containing fluoride exhibited the lowest mean salivary IgA levels, while toothpaste containing CPP-ACP demonstrated the highest levels of sIgA.

1)The CCP-ACP group shows the least levels before application whereas the highest IgA level is seen in the same group post-application.

2)Whereas, in the case of fluoridated toothpaste, the IgA levels pre-application were the highest among the three groups but they reduced drastically after the application.

3)Furthermore, the group treated with nano-hydroxyapatite also exhibited a noteworthy rise in IgA levels subsequent to the application.

Conclusion: Both CPP-ACP and nano-hydroxyapatite-infused toothpaste demonstrated a substantial decrease in salivary IgA levels when compared to toothpaste containing fluoride. When compared to Nano-Hydroxyapatite based toothpaste, CPP-ACP toothpaste showed a better result.