Introduction: Cheiloscopy is a forensic science technique that involves the study of lip prints. The uniqueness of the lip prints have made it an additional tool in personal identification. Like fingerprints, DNA profiling and dental records, lip prints can also be used as an added tool in personal identification. The array of furrows and wrinkles in the red part of the lips is called anatomically as figure line arum labiorum rubrorum, which is unique for each individual 4. Lip prints can be classified into various patterns and can help in criminal investigations, particularly for identifying suspects in cases where lip prints are left as evidence at crime scenes.

Aim: To evaluate various patterns of lip prints in the population and its variation with gender.

Materials and Methods: A random cross-sectional study with a sample size of 95 was conducted in patients who visited the Department of Oral Medicine and Radiology. Recording of lip impressions were performed using dark red lipstick, cellophane strip, magnifying lens, A4 size bond paper. The lip prints recorded were compared with the Suzuki-Tsuchihashi classification.

Result: The predominant pattern obtained among the population was the intersecting or type III pattern of lip print. The type III pattern of lip print was more prevalent in the upper lip and lower lip of both genders.

Conclusion: In the present research, the lip print patterns observed among our study participants were distinct and did not exhibit any significant similarities. Therefore, the result of our research authenticate that lip prints are unique like fingerprints and thus have forensic importance. Although gender -specific lip patterns show substantial differences, conducting studies with larger samples sizes can enhance the accuracy of distinguishing between males and females.