Introduction: The identification of a gender done by barr body which is present in the saliva, blood, tooth, buccal smear, hair, etc. A Barr body is situated at the periphery of the nuclear membrane. Barr body is formed by the inactivation of one of the two female x chromosomes .

Materials and Methods: The study had been conducted on 10 normal females of age 20-26 years. Samples taken from Buccal mucosa, Saliva and Dried saliva from paper. Slides were prepared, stain with Giemsa stain air dry; study under oil immersion lens of compounds microscope.

Results: Buccal swab shows more barr body as compared to other samples.

Conclusion: Buccal cells shows more barr bodies as compared to saliva and least in dried saliva on paper.